Journal: Plant Reproduction
Article Title: The regulation of vacuole morphology in stigma papilla cells is involved in water transfer to pollen in Arabidopsis thaliana
doi: 10.1007/s00497-025-00525-1
Figure Lengend Snippet: The effects of chemical treatment on vacuoles in stigma papilla cells. a Pollen hydration rate at 5 min, 10 min, and 15 min after pollination (n = 15). Error bars indicate mean ± SEM. b – e Representative image sequences of GFP fluorescence in tonoplast of unpollinated 35Spro-TTS::GFP papilla cells treated with mock ( b ), 500 µM E-64d ( c ), 500 µM PDMP ( d ), and 500 µM wortmannin ( e ). Blue arrowheads indicate the site of constriction in central vacuoles. Orange arrows indicate the stacked tubular vacuoles. Purple arrowheads indicate small vacuoles taken up into central vacuoles in the papilla cells treated with PDMP. Yellow arrowheads indicate invagination of the tonoplast in the papilla cells treated with wortmannin. f Number of constrictions of the central vacuoles during 10 min (n = 5). Error bars indicate mean ± SEM. g The time variation of the normalized correlation coefficient with respect to the first image at 10-s intervals (n = 5). The data are presented as in Fig. e. h Time variation of the difference in normalized correlation coefficients with respect to mock treatment in ( g ). Each line and ribbon indicates the median value and 95% credible interval of posterior distributions of the difference for each time period. Statistical results in ( a ) and ( f ) were obtained by Dunnett’s test with the mock treatment as the control group: ** p < 0.01; *** p < 0.001; N.S., not significant
Article Snippet: E-64d ((2s, 3s)- trans -epoxy-succinyl-L-leucylamido-3-methylbutane ethyl ester, Peptide Institute), PDMP (D-L- threo −1-phenyl-3-decanoyl amino-3-morpholino-1-propanol, Enzo Life Sciences), or wortmannin (Fujifilm Wako Pure Chemical Corporation) dissolved in DMSO with 0.025% Tween 20.
Techniques: Fluorescence, Control